EndoBind-R™ is effective over a broad range of conditions with no specific equipment or buffer requirements.
EndoBind-R™ is a DADPA-agarose-conjugated Sushi peptide, affinity chromatography media which exhibits high endotoxin binding affinity (10-7 - 10-8M) and capacity (2,000,000EU/ml of resin).
EndoBind-R™ is chemically synthesized to minimize lot to lot variability, is non-cytotoxic and non-heamolytic, exceptionally stable, and effective over a wide range of pH and ionic conditions.
EndoBind-R™ is fast and simple to use and available as prepacked columns (1ml and 5ml) and as bulk resin
Endotoxin Removal from DNA using EndoBind-R™
Recombinant DNA methodologies have become a powerful tool in many fields of research and in gene therapy. However, DNA purified from gram-negative bacteria is contaminated with very high levels of a pyrogen termed lipopolysaccharide (or endotoxin). To optimize transfection efficiency and reduce adverse reactions to pyrogens, endotoxin must be removed. Here we describe the use of a peptide with high affinity and specificity for endotoxin in column chromatography to produce DNA samples with high recovery and low endotoxin.
Endotoxin Removal from Proteins using EndoBind-R™